Large-scale Screening of Olfactory Sensory Neurons with an Integrated Microfluidic Platform
نویسندگان
چکیده
This paper describes a new platform for the capture, perfusion, and imaging of a large number of dissociated olfactory sensory neurons (OSNs) simultaneously. The platform consists of a fluid selection device integrated with a multiwell plate and a removable-top cell perfusion chamber bonded to an array of addressable cell-trapping microwells. Integration of a 24well plate with our PDMS device allows fluids to be stored on-chip and eliminates the need for external reservoirs and tubing. The removable-top perfusion chamber facilitates cell seeding and enables cell retrieval post-imaging. Finally, an addressable array of microwells allows specific cells of interest to be located following calcium imaging and immunostaining.
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Large-scale investigation of the olfactory receptor space using a microfluidic microwell array.
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